Anti-DNM1L antibody [C7-A4]

Anti-DNM1L antibody [C7-A4] 

• 概述

• 产品描述Dynamin-related protein 1 (DRP1) mediates outer mitochondrial membrane fission in mammalian cells. DRP1 is also known as Dynamin-like protein 1, (Dlp1), DVLP or Dymple. DRP1 contains the N-terminal tripartite GTP-binding domain characteristic of the Dynamin superfamily of GTPases. DRP1 exists as a T-shaped dimer which contains a head, leg and stalk. The addition of GTP induces a rearrangement of the head and stalk that generates a force that ultimately results in membrane constriction. DRP1 is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain. In the cell, DRP1 localized to the perinuclear region. In mouse brain, DRP1 is highly expressed in the cerebellum with particularly high levels in cerebellar Purkinje cells. During apoptosis, DRP1 translocates from the cytosol to mitochondria and localizes to potential sites of organelle division. Cell death is averted upon DRP inhibition, suggesting a critical role for mitochondrial fission in apoptosis.
• 产品名称Anti-DNM1L antibody [C7-A4]
• 分子量82 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • DLP1 antibody
  • dnm1l antibody
  • DNM1L_HUMAN antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of DNM1L on human DNM1L recombinant protein using anti-DNM1L antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of DNM1L on HEK293 (1) and DNM1L-hIgGFc transfected HEK293 (2) cell lysate using anti-DNM1L antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of DNM1L on different cell lysate using anti-DNM1L antibody at 1/1,000 dilution.
  Positive control: Line1: PC-12 Line2: LNCAP Line3: NIH/3T3

   

  

  Fig4: ICC staining DNM1L (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Flow cytometric analysis of HEK293 cells with DNM1L antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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