Anti-ASS1 antibody
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概述
- 产品描述ASS1, also known as argininosuccinate synthase or citrulline-aspartate ligase, belongs to the argininosuccinate synthase family. ASS1 is an urea cycle enzyme with a tetrameric structure composed of identical subunits. It is important to the urea cycle as it catalyzes the important second last step in the arginine biosynthetic pathway. A deficiency of ASS1 causes citrullinemia (CTLN1), an autosomal recessive disease which is characterized by severe vomiting spells and mental retardation.
- 产品名称Anti-ASS1 antibody
- 分子量47 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein A purified.
- 亚细胞定位Cytoplasm.
- 其它名称
- Argininosuccinate synthase 1 antibody
- Argininosuccinate synthase antibody
- Argininosuccinate synthetase 1 antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:100-1:500
IHC-P: 1:50-1:200
FC: 1:50-1:200
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Fig1: Western blot analysis of ASS1 on Hela (1) and MCF-7 (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution.
Fig2: ICC staining ASS1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining ASS1 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ASS1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ASS1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded 2 liver tissue using anti-ASS1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded 2 kidney tissue using anti-ASS1 antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of Jurkat cells with ASS1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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