Anti-Glucocorticoid Receptor alpha antibody
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概述
- 产品描述Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the Glucocorticoid Receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform translocates from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR alpha has a greater affinity for GR response elements (GREs) than GR beta only when in the ligand bound state.
- 产品名称Anti-Glucocorticoid Receptor alpha antibody
- 分子量86/76/60 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC
- 抗体类型兔多抗
- 免疫原Recombinant protein.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein A purified.
- 亚细胞定位Nucleus. Cytoplasm.
- 其它名称
- GCCR antibody
- GCR antibody
- Glucocorticoid receptor alpha isoform antibody
more
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应用
WB: 1:200-1:500
ICC: 1:100-1:500
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Fig1: Western blot analysis of Glucocorticoid Receptor alpha on Hela cell lysate using anti-Glucocorticoid Receptor alpha antibody at 1/1,000 dilution.
Fig2: ICC staining Glucocorticoid Receptor alpha in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Glucocorticoid Receptor alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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