Anti-Occludin antibody
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概述
- 产品描述This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.
- 产品名称Anti-Occludin antibody
- 分子量59 kDa
- 种属反应性Human, Mouse, Rat
- 验证应用WB, ICC, IHC-P, FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human Occludin aa 6-45 conjugated to Keyhole Limpet Haemocyanin (KLH).
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cell membrane.
- 其它名称
- BLCPMG antibody
- FLJ08163 antibody
- FLJ18079 antibody
more
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应用
WB: 1:500-2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of Occludin on mouse kidney (1) and human kidney (2) tissue lysates using anti-Occludin antibody at 1/1,000 dilution.
Fig2: ICC staining Occludin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Occludin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Occludin antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of HepG2 cells with Occludin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
