Anti-SAP97 antibody
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概述
- 产品描述Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.
- 产品名称Anti-SAP97 antibody
- 分子量Predicted band size: 100 kDa.
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种属反应性Human, Mouse
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验证应用WB,IHC-P,FC
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抗体类型兔多抗
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免疫原Synthetic peptide within human aa 1-100.
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偶联Non-conjugated
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性能
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形态Liquid
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浓度1mg/mL
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存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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存储缓冲液1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
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亚型IgG
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纯化方式Peptide affinity purified.
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亚细胞定位Cell junction, Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Synapse.
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其它名称
more
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Discs large homolog 1 antibody
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discs large, Drosophila, homolog of, 1 antibody
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discs, large homolog 1 (Drosophila) antibody
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应用
WB: 1:500
IHC-P:1:50-1:200
FC:1:50-1:100
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Fig1: Western blot analysis of SAP97 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Siha cell lysate
Lane 2: A431 cell lysate
Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-SAP97 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SAP97 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig4: Flow cytometric analysis of SAP97 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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