Anti-Osteocalcin antibody

Anti-Osteocalcin antibody

• 概述

• 产品描述Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein hormone found in bone and dentin, first identified as a calcium-binding protein in chick bone. Because osteocalcin has gla domains, its synthesis is vitamin K dependent. In humans, osteocalcin is encoded by the BGLAP gene. Its receptors include GPRC6A, GPR158, and possibly a third, yet-to-be-identified receptor. Osteocalcin is secreted solely by osteoblasts and thought to play a role in the body's metabolic regulation. In its carboxylated form it binds calcium directly and thus concentrates in bone, but genetic evidence has revealed that it does not play an important role in bone mineralization. In its uncarboxylated form, osteocalcin acts as a hormone in the body, signalling in the pancreas, fat, muscle, testes, and brain. An Acute Stress Response (ASR) (colloquially known as the fight or flight response) stimulates osteocalcin release from bone within minutes in mice, rats, and humans. Injections of high levels of osteocalcin alone can trigger an ASR in the presence of adrenal insufficiency.
• 产品名称Anti-Osteocalcin antibody
• 分子量11 kDa
• 种属反应性Human, Mouse, Rat, Dog, Pig, Cow, Horse
• 验证应用WB, IHC-P, ICC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human Osteocalcin:21-100/100
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/ml.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Affinity purified by Protein A
• 亚细胞定位Secreted.
• 其它名称
  • BGLAP antibody
  • BGP antibody
  • Bone gamma carboxyglutamate (gla) protein antibody
  more

• 应用

  WB: 1:500-1:2000
  IHC-P: 1:50-1:200
  ICC: 1:50-1:200

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig2: Citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin  at [1:500] for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium clei.

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