Anti-POLR3H antibody
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概述
- 产品描述DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with CRCP/RPC9. POLR3H/RPC8 and CRCP/RPC9 probably form a Pol III subcomplex.
- 产品名称Anti-POLR3H antibody
- 分子量23 kDa
- 种属反应性Human, Mouse, Rat
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human RPC8. AA range:151-200
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称
- POLR3H antibody; KIAA1665 antibody; RPC8 antibody; DNA-directed RNA polymerase III subunit RPC8 antibody; RNA polymerase III subunit C8 antibody; DNA-directed RNA polymerase III subunit H antibody; RNA polymerase III subunit 22.9 kDa subunit antibody; RPC22.9 antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Western Blot analysis of K562 cells using POLR3H Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Fig2: Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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