Anti-Nestin antibody

Anti-Nestin antibody

• 概述

• 产品描述Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differen-tiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.

• 产品名称Anti-Nestin antibody

• 分子量177 kDa(Predicted band size)

• 种属反应性Human,Mouse

• 验证应用ICC

• 抗体类型兔多抗

• 免疫原Peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cytoskeleton, extracellular region or secreted.

• 其它名称

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• 应用

  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100
  WB: 1:500

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  Fig1: ICC staining Nestin in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: ICC staining Nestin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Nestin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Nestin antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Nestin antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of N2A cells with Nestin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

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