Anti-TRIM68 antibody
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概述
- 产品描述Functions as an ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity.
- 产品名称Anti-TRIM68 antibody
- 分子量56 KDa
- 种属反应性Human,Mouse,Rat,Pig,Cow,Horse,Sheep
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human TRIM68 201-300/485
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cytoplasm > perinuclear region. Nucleus. Colocalized with AR in nucleus.
- 其它名称E3 ubiquitin-protein ligase TRIM68
FLJ10369
GC109
MGC126176
RING finger protein 137
RNF 137
RNF137
Ro/SSA1 related protein
SS 56
SS-56
SS56
SSA protein SS 56
SSA protein SS-56
TRI68_HUMAN
TRIM 68
Trim68
Tripartite motif containing 68
Tripartite motif containing protein 68
Tripartite motif-containing protein 68.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TRIM68) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
HepG2(Human) Cell Lysate at 40 ug
Primary: Anti-TRIM68at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
