Anti-VCP antibody

Anti-VCP antibody

• 概述

• 产品描述Valosin-containing protein (VCP) is a highly conserved, that belongs to the AAA (ATPase associated with a variety of cellular activities) family of proteins. It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. VCP is involved in the formation of the transitional endoplasmic reticulum (tER). VCP homo-hexamers associate with a variety of protein cofactors to form many distinct protein complexes, which act as chaperones to unfold proteins and transport them to specific cellular compartments or to the proteosome.
• 产品名称Anti-VCP antibody
• 分子量89 kDa
• 种属反应性Human,Mouse,Zebrafish
• 验证应用WB,ICC,IHC-P
• 抗体类型兔多抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified
• 亚细胞定位Cytoplasm, nucleus, endoplasmic reticulum
• 其它名称15S Mg(2+) ATPase p97 subunit antibody
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  ALS14 antibody
  ATPase p97 antibody
  CDC48 antibody
  IBMPFD antibody
  MGC131997 antibody
  MGC148092 antibody
  MGC8560 antibody
  p97 antibody
  TER ATPase antibody
  TERA antibody
  TERA_HUMAN antibody
  Transitional endoplasmic reticulum ATPase antibody
  Valosin containing protein antibody
  Valosin-containing protein antibody
  VCP antibody
  Yeast Cdc48p homolog antibody
  more

• 应用

  WB: 1:2,000
  ICC: 1:200
  IHC-P: 1:200

•  

  

  Fig1: Western blot analysis of VCP on different cell lysates using anti- VCP antibody at 1/2000 dilution.
  Positive control:
  Lane 1: L929
  Lane 2: F9
  Lane 3: A549
  Lane 4: MCF-7
  Lane 5: Hela
  Lane 6: NIH/3T3
  Lane 7: N2A
  Lane 8: Jurkat
  Lane 9: HepG2
  Lane 10: A431
  Lane 11: Mouse heart
  Lane 12: Mouse brain
  Lane 13: Mouse liver
  Lane 14: Mouse kidney

   

  

  Fig2: ICC staining VCP in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining VCP in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining VCP in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: ICC staining VCP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-VCP antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-VCP antibody. Counter stained with hematoxylin.

   

  

  Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-VCP antibody. Counter stained with hematoxylin.

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