Anti-ATG16L1 antibody [A11-H9]

Anti-ATG16L1 antibody [A11-H9]

• 概述

• 产品描述Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.
• 产品名称Anti-ATG16L1 antibody [A11-H9]
• 分子量68 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
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• 其它名称
  • A16L1_HUMAN antibody
  • APG16 like 1 antibody
  • APG16-like 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:100-1:200

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  Fig1: Western blot analysis of ATG16L1 on human ATG16L1 recombinant protein using anti-ATG16L1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ATG16L1 on HEK293 (1) and ATG16L1-hIgGFc transfected HEK293 (2) cell lysate using anti-ATG16L1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of ATG16L1 on different cell lysate using anti-ATG16L1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: Raji
  Lane 3: PANC-1
  Lane 4: Jurkat
  Lane 5: PC-12
  Lane 6: HepG2
  Lane 7: Hek293
  Lane 8: NIH3T3

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