Anti-MADH7/Smad7 antibody

Anti-MADH7/Smad7 antibody

• 概述

• 产品描述Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

• 产品名称Anti-MADH7/Smad7 antibody

• 分子量46 KDa

• 种属反应性Human,Mouse,Rat,Pig,Cow

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Smad7 1-100/426

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 or induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytopl

   

• 其它名称hSMAD 7
  hSMAD7
  MAD (mothers against decapentaplegic Drosophila) homolog 7
  MAD
  Mad homolog 7
  MAD mothers against decapentaplegic homolog 7
  MADH 7
  MADH 8
  MADH8
  Mothers Against Decapentaplegic Drosophila Homolog of 7
  Mothers against decapentaplegic homolog 7
  Mothers against decapentaplegic homolog 8
  Mothers against DPP homolog 7
  Mothers against DPP homolog 8
  SMA- AND MAD-RELATED PROTEIN 7
  SMAD 7
  SMAD
  SMAD family member 7
  SMAD, mothers against DPP homolog 7 (Drosophila)
  SMAD, mothers against DPP homolog 7
  Smad7
  SMAD7_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  Lung(Mouse) Lysate at 30 ug
  Placenta(Mouse) Lysate at 30 ug
  Large intestine(Mouse) Lysate at 30 ug
  Primary: Anti- MADH7/Smad7 (at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 46 kD
  Observed band size: 50 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Smad7) Polyclonal Antibody, Unconjugated  at 1:600 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig3: Sample:Lymph node (Mouse) Lysate at 30 ug
  Primary: Anti- MADH7/Smad7  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 46 kD
  Observed band size: 50 kD

   

  

  Fig4: Sample:Spleen(Mouse) Lysate at 30 ug
  Primary: Anti- MADH7/Smad7  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 46 kD
  Observed band size: 50 kD

   

  

  Fig5: Sample:Stomach (Mouse) Lysate at 30 ug
  Primary: Anti- MADH7/Smad7  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 46 kD
  Observed band size: 50 kD

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