Anti-PDE7B antibody

Anti-PDE7B antibody

• 概述

• 产品描述PDE7B is a cAMP specific phosphodiesterase. The cAMP-specific phosphodiesterase type-7 (PDE7) family is comprised of 2 genes (PDE7A and PDE7B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. PDE7B has three splice variants (PDE7B1 = 446 amino acid; PDE7B2 =359 amino acids and PDE7B3 = 459 amino acids. Both PDE7B2 ad PDE7B3 posses unique N-terminal sequences. PDE7B is expressed in various tissues including skeletal muscle, heart, lung and testis. PDE7B may be involved in the control of cAMP-mediated neural activity and cAMP metabolism in the brain.

• 产品名称Anti-PDE7B antibody

• 分子量52 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PDE7B 281-360/450

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

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• ​其它名称PDE 7B
  PDE7B protein
  Phosphodiesterase 7B
  Phosphodiesterase7B
  RNPDE7B
  Rolipram insensitive phosphodiesterase type 7
  bA472E5.1
  cAMP specific 3 5 cyclic phosphodiesterase 7B
  cAMP specific 3'5' cyclic phosphodiesterase 7B
  High affinity cAMP specific 3 5 cyclic phosphodiesterase
  High affinity cAMP specific 3'5' cyclic phosphodiesterase
  PDE7B_HUMAN.

  more

• 应用

  WB:1:500-2000
  FC:1μg/Test

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  Fig1: Sample:
  Brain (Mouse) Lysate at 40 ug
  Liver (Mouse) Lysate at 40 ug
  Primary: Anti-PDE7B  at 1/300 dilution
  Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
  Predicted band size: 52 kD
  Observed band size: 52 kD

   

  

  Fig2: Blank control(blue): RSC96 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
  Primary Antibody:Rabbit Anti- PDE7B antibody( Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

   

  

  Fig3: Sample: Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti-PDE7Bat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 52 kD
  Observed band size: 52 kD

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