Anti-HO-2 antibody

Anti-HO-2 antibody

• 概述

• 产品描述Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

• 产品名称Anti-HO-2 antibody

• 分子量36 KDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human HO-2 31-150/316

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Microsome. Endoplasmic reticulum.

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• ​其它名称Heme oxygenase (decycling) 2
  Heme oxygenase (decyclizing) 2
  HMOX 2
  HMOX2
  HMOX2 protein
  HO 2
  HO2
  HMOX2_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:0.2ug/test

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HO-2) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample:
  Brain(Rat)lysate at 30ug;
  Liver(Rat) lysate at 30ug;
  Primary: Anti-HO-2  at 1:200 dilution;
  Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
  Predicted band size : 38kD
  Observed band size : 38kD, 48kD

   

  

  Fig3: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HO-2 Antibodyat 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

   

  

  Fig4: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti- HO-2 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 36 kD
  Observed band size: 33 kD

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