Anti-ILVBL antibody
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概述
- 产品名称Anti-ILVBL antibody
- 分子量68 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Horse
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human ILVBL 301-400/632
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Membrane.
- 其它名称Acetolactate synthase homolog
Acetolactate synthase like protein
Acetolactate synthase-like protein
AHAS
ILV2H
209L8
IlvB (bacterial acetolactate synthase) like
IlvB like protein
IlvB-like protein
Ilvbl
ILVBL_HUMAN.
more
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应用
WB:1:500-2000
FC:3ug/Test
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Fig1: Sample: HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-ILVBL at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68kD
Observed band size: 68kD
Fig2: Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-ILVBL antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig3: Sample:
Hcclm3(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Primary: Anti- ILVBL at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
