Anti-CKMT antibody

Anti-CKMT antibody

• 概述

• 产品描述Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

• 产品名称Anti-CKMT antibody

• 分子量43 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CKMT 115-220/417

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

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• ​其它名称Acidic type mitochondrial creatine kinase
  CKMT
  CKMT1
  CKMT1A
  CKMT1B
  Creatine kinase mitochondrial 1 ubiquitous
  Creatine kinase ubiquitous mitochondrial
  Mia CK
  Ubiquitous mitochondrial creatine kinase
  UMTCK
  KCRU_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-CKMT/Creatine kinase MT Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig2: Sample:
  Small intestine (Mouse) Lysate at 40 ug
  Primary: Anti-CKMT (at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 43 kD
  Observed band size: 43 kD

   

  

  Fig3: Sample:
  Large intestine (Rat) Lysate at 40 ug
  Primary: Anti-CKMTat 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 43 kD
  Observed band size: 43 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！