Anti-Phospho-eIF-2a (Ser 52) antibody
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概述
- 产品描述The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2, eIF2 and eIF2 (eukaryotic translation initiation factor 2 , and , respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2 controls the rate of tRNA translation. When eIF2 is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2 is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.
- 产品名称Anti-Phospho-eIF-2a (Ser 52) antibody
- 分子量36 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IP,IF,IHC-P
- 抗体类型兔多抗
- 免疫原A short amino acid sequence containing phosphorylated Ser 52 of eIF2α of human origin.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Cytoplasmic granule
- 其它名称
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
more
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应用
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
IF: 1:50-1:500
IHC-P: 1:50-1:500
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Fig1: Western blot analysis of eIF2α phosphorylation in untreated (A, C) and lambda protein phosphatase treated (B, D) HEK293 whole cell lysates. Antibodies tested include p-eIF2α (Ser 52)(A, B) and eIF2α(C, D).
Fig2: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.
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