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货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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储存条件: | -20℃ |
Anti-TRAIL antibody
WB:1:500-2000
IHC-P:1:400-800
Fig1: Sample:
Lung (Mouse) Lysate at 30 ug
U251 Cell Lysate at 30 ug
Primary: rabbit Anti- TRAIL at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1:5000 dilution;
Predicted band size: 31 kD
Observed band size: 26 kD
Fig2: Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig3: Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated() 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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