Anti-HFH4 antibody

Anti-HFH4 antibody

• 概述

• 产品描述May play an important role in cell fate determination during lung development and in spermatogenesis.

• 产品名称Anti-HFH4 antibody

• 分子量46 KDa

• 种属反应性Human,Mouse,Rat,Dog,Cow,Rabbit

• 验证应用WB,IHC-P,ICC/IF,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human FOXJ1 161-260/421

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus.

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• ​其它名称Forkhead box protein J1
  FOXJ-1
  winged helix
  FOXJ1
  FKHL13
  HFH-4
  HFH4
  MGC35202
  forkhead box J1
  Forkhead Homologue 4
  Forkhead related protein FKHL13
  Fox J1
  Hepatocyte nuclear factor 3 forkhead homolog 4
  HNF-3/forkhead homolog 4
  FOXJ1_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:0.2ug/test
  IF:1:100-500

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample:
  Testis (Mouse) Lysate at 40 ug
  Testis (Rat) Lysate at 40 ug
  Primary: Anti-HFH4  at 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 46 kD
  Observed band size: 50 kD

   

  

  Fig3: Sample:
  Testis (Mouse) Lysate at 40 ug
  Testis (Rat) Lysate at 40 ug
  Primary: Anti-HFH4  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 46 kD
  Observed band size: 50 kD

   

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig5: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated ( at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig6: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig7: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HFH4 Antib

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