Anti-CBX4 antibody

Anti-CBX4 antibody

• 概述

• 产品描述E3 SUMO-protein ligase which facilitates SUMO1 conjugation by UBE2I. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.

• 产品名称Anti-CBX4 antibody

• 分子量61 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Horse,Sheep

• 验证应用WB

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CBX4 401-500/560

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus.

   

• 其它名称CBX 4
  CBX4
  Cbx4 chromobox homolog 4 (Drosophila Pc class)
  CBX4_HUMAN
  Chromobox homolog 4 (Pc class homolog, Drosophila)
  Chromobox homolog 4
  Chromobox protein homolog 4
  E3 SUMO protein ligase CBX 4
  E3 SUMO protein ligase CBX4
  E3 SUMO-protein ligase CBX4
  hPc 2
  hPc2
  NBP 16
  NBP16
  NS5ATP1 binding protein 16
  Pc 2
  Pc class 2 homolog
  Pc class homolog
  Pc class homolog Drosophila
  PC2
  Polycomb 2 homolog.

  more

• 应用

  WB:1:500-2000

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  Fig1: Sample:
  Testis(Mouse) Cell Lysate at 40 ug
  Hepg2(Human) Cell Lysate at 30 ug
  Primary: Anti-CBX4  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 42 kD
  Observed band size: 42 kD

   

  

  Fig2: Sample:
  Adrenal gland (Mouse) Lysate at 40 ug
  Jurkat(Human) Cell Lysate at 30 ug
  Testis (Mouse) Lysate at 40 ug
  HepG2(Human) Cell Lysate at 30 ug
  Primary: Anti- CBX4 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 61 kD
  Observed band size: 63 kD

   

  

  Fig3: Sample:
  K562(Human) Cell Lysate at 30 ug
  MCF-7(Human) Cell Lysate at 30 ug
  Primary: Anti- CBX4at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 61 kD
  Observed band size: 61 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！