Anti-Cathepsin B antibody

Anti-Cathepsin B antibody

• 概述

• 产品描述Cathepsin B is synthesized on the rough endoplasmic reticulum as a preproenzyme of 339 amino acid with a signal peptide of 17 amino acids. Procathepsin B of 43/46 kDa is then transported to the Golgi apparatus and cathepsin B is formed. Mature cathepsin B is composed of a heavy chain of 25-26 kDa and a light chain of 5kDa, which are linked by a dimer of disulfide. Cathepsin B may enhance the activity of other protease, including matrix metalloproteinase, urokinase (serine protease urokinase plasminogen activator), and cathepsin D, and thus it has an essential position for in the proteolysis of extracellular matrix components, intercellular communication disruption, and reduced protease inhibitor expression. It is also involved in autophagy and catabolism, which is advantageous in tumor malignancy and is possibly involved in specific immune resistance.

• 产品名称Anti-Cathepsin B antibody

• 分子量23/28/37 KDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Cathepsin B heavy chain 251-339/339

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

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• ​其它名称Amyloid precursor protein secretase
  APP secretase
  APPS
  Cathepsin B
  CB
  Cathepsin B1
  CathepsinB
  CPSB
  CTSB
  Cysteine protease
  Preprocathepsin B
  Cathepsin B heavy chain
  Cathepsin B light chain
  CATB_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1µg/Test

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  Fig1: Sample:
  Spleen (Mouse) Lysate at 40 ug
  Primary: Anti-Cathepsin B  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 32 kD
  Observed band size: 32 kD

   

  

  Fig2: Blank control: RSC96(blue).
  Primary Antibody:Rabbit Anti- Cathepsin B antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) . Antibody ( 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody ofat 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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