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| 别名: | KCNH1 | ||
|---|---|---|---|
| 适用物种: | Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit | ||
| 验证应用: | WB,FC | ||
| 种属: | 兔多抗 | ||
| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Anti-KCNH1 antibody
产品描述Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts.
产品名称Anti-KCNH1 antibody
分子量111 KDa
种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human KCNH1 501-600/989
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Membrane; Multi-pass membrane protein.
其它名称
WB:1:500-2000
FC:1μg/Test
Fig1: Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti- KCNH1 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD
Fig2: Blank control: Hela(blue).
Primary Antibody:Rabbit Anti-KCNH1 antibody(ER1911-71), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-2442R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-2442R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
