Anti-FH antibody [7F1]

Anti-FH antibody [7F1]

• 概述

• 产品描述The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
• 产品名称Anti-FH antibody [7F1]
• 分子量50 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, IHC-P, IF
• 抗体类型小鼠单抗
• 免疫原Synthetic Peptide of FH
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
• 纯化方式Immunogen affinity purified.
• 其它名称
  • Fumarate hydratase, mitochondrial (Fumarase) (EC 4.2.1.2) antibody;

• 应用

  WB: 1:3000
  IF: 1:200
  IHC-P: 1:50-300

•  

  

  Fig1: Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,FH Monoclonal Antibody(7F1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

   

  

  Fig2: Immunofluorescence analysis of Human-liver-cancer tissue. 1,FH Monoclonal Antibody(7F1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

   

  

  Fig3: Western blot analysis of 1) 293T, 2) HepG2, 3) Hela, diluted at 1:3000.

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