Anti-ACCN2 antibody

Anti-ACCN2 antibody

• 概述

• 产品描述Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.Isoform 1 does not display proton-gated cation channel activity.

• 产品名称Anti-ACCN2 antibody

• 分子量60 kDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原Synthetic peptide within human ACCN2 aa 460-500.

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified.

• 亚细胞定位Plasma membrane.

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• ​其它名称ACCN2 antibody
  ACCN2_HUMAN antibody
  Acid sensing ion channel 1 antibody
  Acid sensing ion channel 1a protein antibody
  Acid sensing ion channel antibody
  Acid-sensing ion channel 1 antibody
  AI843610 antibody
  Amiloride sensitive cation channel 2 neuronal antibody
  Amiloride-sensitive cation channel 2 antibody
  ASIC antibody
  ASIC1 antibody
  ASIC1A antibody
  B530003N02Rik antibody
  BNaC2 antibody
  Brain sodium channel 2 antibody
  Cation channel amiloride sensitive neuronal 2 antibody
  hBNaC2 antibody
  neuronal antibody

  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

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  Fig1: Western blot analysis of ACCN2 on SH-SY-5Y cell lysate using anti-ACCN2 antibody at 1/2,000 dilution.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of SH-SY-5Y cells with ACCN2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！