Anti-EID-1 antibody
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概述
- 产品描述developmental stage:Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level). Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms. induction:Down-regulated in differentiating U937 leukemia cells. miscellaneous:Inhibition of MYOD1 may be partly due to the ability of EID1 to bind and inhibit EP300 histone acetyltransferase activity. PTM:Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit. subcellular location:May shuttle between nucleus and cytoplasm. subunit:Interacts via its LXCXE motif with the entire pocket region of RB1. Interacts with EP300, NR0B2 and TRIM27. tissue specificity:Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines.
- 产品名称Anti-EID-1 antibody
- 分子量21 kDa
- 种属反应性Human
- 验证应用WB, IHC-P, IF
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human EID1. AA range:71-120
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称EID1 antibody; C15orf3 antibody; CRI1 antibody; RBP21 antibody; PNAS-22 antibody; PTD014 antibody; EP300-interacting inhibitor of differentiation 1 antibody; 21 kDa pRb-associated protein antibody; CREBBP/EP300 inhibitory protein 1 antibody; E1A-like inhibitor of differentiation 1 antibody; EID-1 antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
IF: 1:100-1:500
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Fig1: Western Blot analysis of various cells using EID-1 Polyclonal Antibody
Fig2: Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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