Anti-UAP1 antibody
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概述
- 产品描述Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P. This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.
- 产品名称Anti-UAP1 antibody
- 分子量58 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human UAP1 380-430 aa.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cytoplasm.
- 其它名称AGX 1 antibody
AGX antibody
AGX-1 antibody
AGX-2 antibody
AGX1 antibody
Antigen X antibody
AntigenX antibody
SPAG 2 antibody
SPAG2 antibody
Sperm associated antigen 2 antibody
Sperm-associated antigen 2 antibody
UAP 1 antibody
uap1 antibody
UAP1_HUMAN antibody
UDP N acetylhexosamine pyrophosphorylase antibody
UDP N acteylglucosamine pyrophosphorylase 1 antibody
UDP-N-acetylglucosamine pyrophosphorylase antibody
more
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应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of UAP1 on SiHa cell lysate using anti-UAP1 antibody at 1/500 dilution.
Fig2: ICC staining UAP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining UAP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-UAP1 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of K562 cells with UAP1 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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