Anti-Dopamine Transporter antibody

Anti-Dopamine Transporterantibody

• 概述

• 产品描述The dopamine transporter (also dopamine active transporter, DAT, SLC6A3) is a membrane-spanning protein that pumps the neurotransmitter dopamine out of the synaptic cleft back into cytosol. In the cytosol, other transporters sequester the dopamine into vesicles for storage and later release. Dopamine reuptake via DAT provides the primary mechanism through which dopamine is cleared from synapses, although there may be an exception in the prefrontal cortex, where evidence points to a possibly larger role of the norepinephrine transporter. DAT is implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder, bipolar disorder, clinical depression, alcoholism, and substance use disorder. The gene that encodes the DAT protein is located on human chromosome 5, consists of 15 coding exons, and is roughly 64 kbp long. Evidence for the associations between DAT and dopamine related disorders has come from a type of genetic polymorphism, known as a VNTR, in the DAT gene (DAT1), which influences the amount of protein expressed. Dopamine underlies several aspects of cognition, including reward, and DAT facilitates regulation of that signal.
• 产品名称Anti-Dopamine Transporter antibody
• 分子量68 KDa
• 种属反应性Human,Mouse,Rat,Dog,Cow
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human DAT1 251-350/620
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式Affinity purified by Protein A
• 亚细胞定位Membrane; Multi-pass membrane protein.
• 其它名称DA transporter
  DAT 1
  DAT
  DAT1
  Dopamine transporter
  SC6A3_HUMAN
  SLC6A3
  Sodium dependent dopamine transporter
  Sodium-dependent dopamine transporter
  Solute carrier family 6 (neurotransmitter transporter dopamine), member 3
  Solute carrier family 6 member 3
  Variable number tandem repeat (VNTR)
  dopamine transporter
  ADAT 1
  Adenosine deaminase tRNA specific 1
  HADAT1
  tRNA specific adenosine deaminase 1.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:0.2μg /test

•  

  

  Fig1: Positive control: RSC96
  Isotype Control Antibody: Rabbit IgG; Secondary Antibody: Goat anti-rabbit IgG-FITC; Dilution: 1:200 in 1 X PBS containing 0.5% BSA
  Primary Antibody catalog number: ; Dilution: 12μg in 100 μl 1X PBS containing 0.5% BSA

   

  

  Fig2: Positive control: RSC96
  Isotype Control Antibody: rabbit IgG-PE/CY5, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 6μg in 100 μL1X PBS containing 0.5% BSA.

   

  

  Fig3: Blank control: U937 (blue).
  Primary Antibody:Rabbit Anti-Dopamine Transporter antibodyDilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

   

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated ) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig5: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti-Dopamine Transporterat 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 68 kD
  Observed band size: 68 kD

   

  

  Fig6: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine Transporter) Polyclonal Antibody, Unconjugated ( at 1:400 overnight at 4℃, followed by operating according to SP Kit (Rabbit) (sp-0023) instructionsand DAB staining.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！