Anti-Frizzled 10/CD350 antibody
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概述
- 产品描述Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
- 产品名称Anti-Frizzled 10/CD350 antibody
- 分子量68 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Sheep
- 验证应用WB
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human Frizzled 10/CD350 101-200/581
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane.
- 其它名称CD 350
CD 350 antigen
CD350
Frizzled (Drosophila) homolog 10
Frizzled-10
FZ 10
Fz-10
FZ10
FZD 10
FZD10
FZD10_HUMAN
FzE 7
FzE7
hFz 10
hFz10.
more
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Fig1: Protein:
embryo(mouse) lysates at 30ug;
colon carcinoma(human) lysates at 30ug;
Primary: Anti-Frizzled 10/CD350 at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 68kD
Observed band size : 68kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
