Anti-EphA7 antibody
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概述
- 产品描述’Receptor tyrosine kinase which binds promiscuouslyGPI-anchored ephrin-A family ligands residing on adjacent cells,leading to contact-dependent bidirectional signaling intoneighboring cells. The signaling pathway downstream of the receptoris referred to as forward signaling while the signaling pathwaydownstream of the ephrin ligand is referred to as reversesignaling. Among GPI-anchored ephrin-A ligands, EFNA5 is acognate/functional ligand for EPHA7 and their interaction regulatesbrain development modulating cell-cell adhesion and repulsion. Hasa repellent activity on axons and is for instance involved in theguidance of corticothalamic axons and in the proper topographicmapping of retinal axons to the colliculus. May also regulate braindevelopment through a caspase(CASP3)-dependent proapoptoticactivity. Forward signaling may result in activation of componentsof the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 ANDMAPK3 which are phosphorylated upon activation of EPHA7. Isoform 4which lacks the kinase domain may regulate isoform 1 adhesiveproperties.
- 产品名称Anti-EphA7 antibody
- 分子量50/107 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Dog,Cow,Horse,Rabbit
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human EphA7 181-280/998
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane (Probable); Single-pass type I membrane protein (Probable).
- 其它名称Cek 11
Developmental kinase 1
EBK
EHK-3
EHK3
EK11
Embryonic brain kinase
Eph homology kinase 3
Eph receptor A7
EPH-like kinase 11
EphA7
EPHA7_HUMAN
Ephrin receptor Eph A7
Ephrin type A receptor 7
Ephrin type-A receptor 7
hEK11
MDK 1
Receptor protein tyrosine kinase HEK 11
Tyrosine protein kinase receptor EHK 3.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-EphA7at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/107 kD
Observed band size: 52 kD
Fig2: Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-EphA7at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50/107 kD
Observed band size: 52 kD
Fig3: Protein: rat brain lysates, 30ug;
Primary: Anti-EphA7 at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 107kD
Observed band size : 45kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
