Anti-ZAR1L antibody

Anti-ZAR1L antibody

• 概述

• 产品名称Anti-ZAR1L antibody
• 分子量36 KDa
• 种属反应性Human
• 验证应用IHC-P,WB
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human ZAR1L 51-150/321
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 其它名称RP11-37E23.4
  ZAR1-like protein
  ZAR1L
  ZAR1L_HUMAN
  ZAR2
  Zygote arrest 1 like.

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:A549 (Human)cell Lysate at 40 ug
  Primary: Anti-ZAR1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
  Predicted band size: 36kD
  Observed band size: 36kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ZAR1L) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！