Anti-GAP43 antibody

Anti-GAP43 antibody

• 概述

• 产品描述GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts. It is a major protein kinase C (PKC) substrate and is considered to play a key role in neurite formation, regeneration, and plasticity. The role of GAP-43 in CNS development is not limited to effects on axons: It is also a component of the centrosome, and differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. As a consequence, in the cerebellum, the neuronal precursor pool fails to expand normally and the cerebellum is significantly smaller.

• 产品名称Anti-GAP43 antibody

• 分子量43 kDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cell membrane.

   

• 其它名称Axonal membrane protein GAP 43 antibody
  Axonal membrane protein GAP-43 antibody
  B 50 antibody
  Calmodulin binding protein P 57 antibody
  F1 antibody
  GAP 43 antibody
  GAP43 antibody
  Growth Associated Protein 43 antibody
  Growth-associated protein 43 antibody
  Nerve Growth Related Peptide antibody
  Nerve growth related peptide GAP43 antibody
  NEUM_HUMAN antibody
  Neural phosphoprotein B 50 antibody
  Neural phosphoprotein B-50 antibody
  Neuromodulin antibody
  Neuron growth associated protein 43 antibody
  PP46 antibody
  Protein F1 antibody
  QtrA-11580 antibody
  QtrA-13071 antibody

  more

• 应用

  WB: 1:1,000
  ICC: 1:200
  IHC-P: 1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of GAP43 on different cell lysates using anti-GAP43 antibody at 1/1000 dilution.
  Positive control:
  Lane 1: Rat brain
  Lane 2: Mouse brain
  Lane 3: Mouse heart
  Lane 4: Human skeletal muscle
  Lane 5: N2A
  Lane 6: A172
  Lane 7: Human heart

   

  

  Fig2: ICC staining GAP43 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining GAP43 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining GAP43 in SHG-44 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: ICC staining GAP43 in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GAP43 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GAP43 antibody. Counter stained with hematoxylin.

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