Anti-NFkB Inducing Kinase NIK antibody

Anti-NFkB Inducing Kinase NIK antibody

• 概述

• 产品描述Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
• 产品名称Anti-NFkB Inducing Kinase NIK antibody
• 分子量104 KDa
• 种属反应性Human,Mouse,Rat,Dog,Cow,Horse,Rabbit
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human NIK 901-960/947
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm.
• 其它名称NF-kappaB-Inducing Kinase
  NFkB Inducing Kinase NIK
  NF kappa beta inducing kinase
  NF-kappa-beta-inducing kinase
  HS
  HSNIK
  NIK
  Serine/threonine-protein kinase NIK
  mitogen-activated protein kinase kinase kinase 14
  MAPKKK14
  MAP3K14
  FTDCR1B
  NF-kappa-beta-inducing kinase
  M3K14_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:3ug/Test

•  

  

  Fig1: Sample:
  Kidney(Mouse) lysate at 30ug;
  Brain(Mouse) lysate at 30ug
  Primary: Anti-NFkB Inducing Kinase NIK at 1:300 dilution;
  Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
  Predicted band size : 104kD
  Observed band size : 104kD

   

  

  Fig2: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-HSNIK Polyclonal Antibody, Unconjugated1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig3: Blank control: A431.
  Primary Antibody (green line): Rabbit Anti-NFkB Inducing Kinase NIK antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody: Goat anti-rabbit IgG-AF647
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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