Anti-EGFR5 antibody
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概述
- 产品名称Anti-EGFR5 antibody
- 分子量49 KDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human EGFR5/CLEC14A 221-320/490
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Membrane; Single pass type I membrane protein.
- 其它名称C type lectin domain family 14, member A
C14orf27
CEG1
ClECT and EGF like domain containing protein
EGFR5
EGFR-5
EGFR 5
Epidermal growth factor receptor 5
CLC14_HUMAN.
more
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应用
WB:1:500-2000
FC:1μg/Test
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Fig1: Sample:
B16 Cell (Mouse) Lysate at 40 ug
DU145 Cell (Human) Lysate at 40 ug
Primary: Anti-EGFR5 ( at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 53 kD
Fig2: Blank control(blue):293T (fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-EGFR5 antibodyDilution: 1μg in 100μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA
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