Anti-VAX1 antibody

Anti-VAX1 antibody

• 概述

• 产品描述Required for axon guidance and major tract formation in the developing forebrain. May contribute to the differentiation of the neuroretina, pigmented epithelium and optic stalk.
• 产品名称Anti-VAX1 antibody
• 分子量35 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human VAX1 133-200/334
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Nucleus.
• 其它名称VAX1
  VAX1_HUMAN
  ventral anterior homeobox 1.

• 应用

  WB:1:500-2000
  FC:0.2μg/Test

•  

  

  Fig1: Sample: RAW264.7 Cell (Mouse) Lysate at 40 ug
  Primary: Anti- VAX1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 35 kD
  Observed band size: 37 kD

   

  

  Fig2: Sample: BRL-3A Cell (Rat) Lysate at 40 ug
  Primary: Anti- VAX1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 35 kD
  Observed band size: 37 kD

   

  

  Fig3: Sample: NIH/3T3 Cell (Mouse) Lysate at 40 ug
  Primary: Anti- VAX1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 35 kD
  Observed band size: 37 kD

   

  

  Fig4: Sample:
  MCF-7 Cell (Human) Lysate at 40 ug
  U937 Cell (Human) Lysate at 40 ug
  Primary: Anti-VAX1 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 35 kD
  Observed band size: 36 kD

   

  

  Fig5: Blank control (blue line): Hep G2 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% methanol for 20 min at -20℃).
  Primary Antibody (green line): Rabbit Anti-VAX1 antibodyDilution: 0.2μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.

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