Anti-PRKAA2 antibody [H5-E11]

Anti-PRKAA2 antibody [H5-E11]

• 概述

• 产品描述The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
• 产品名称Anti-PRKAA2 antibody [H5-E11]
• 分子量62 kDa
• 种属反应性Human,Monkey
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus.
• 其它名称
  • 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody
  • AAPK2_HUMAN antibody
  • ACACA kinase antibody
  more

• 应用

  WB: 1:500
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

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  Fig1: Western blot analysis of AMPK alpha 2 on human AMPK alpha 2 recombinant protein using anti- AMPK alpha 2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of AMPK alpha 2 on HEK293 (1) and AMPK alpha 2-hIgGFc transfected HEK293 (2) cell lysate using anti- AMPK alpha 2 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of AMPK alpha 2 on HEK293 and COS7 cell lysate using anti- AMPK alpha 2 antibody at 1/1,000 dilution.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti- AMPK alpha 2 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti- AMPK alpha 2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Jurkat cells with AMPK alpha 2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！