Anti-Dynamin 3 antibody
-
概述
- 产品描述Dynamin-3 is a protein that in humans is encoded by the DNM3 gene. The protein encoded by this gene is a member of the dynamin family which possess mechanochemical properties involved in actin-membrane processes, predominantly in membrane budding. DNM3 is upregulated in Sézary's syndrome.Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis (By similarity).
- 产品名称Anti-Dynamin 3 antibody
- 分子量Predicted band size: 97 kDa.
- 种属反应性Human,Rat
- 验证应用WB,ICC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human Dynamin 3 aa 750-800.
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cytoplasm, Cytoskeleton, Microtubule.
- 其它名称
- testicular dynamin antibody
- testicular antibody
- DNM 3 antibody
more
-
应用
WB: 1:500
ICC: 1:50-1:200
-
Fig1: Western blot analysis of Dynamin 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat brain tissue lysate
Lane 2: PC12 cell lysate
Fig2: ICC staining of Dynamin 3 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
