Anti-MARCH9 antibody
-
概述
- 产品描述E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I, CD4 and ICAM1, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
- 产品名称Anti-MARCH9 antibody
- 分子量38 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human MARCH9 201-300/346
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Golgi apparatus membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein.
- 其它名称E3 ubiquitin protein ligase MARCH9
MARCH IX
Membrane associated RING CH protein IX
membrane associated ring finger (C3HC4) 9
Membrane associated RING finger protein 9
RING finger protein 179
RNF179
MARH9_HUMAN.
more
-
应用
WB:1:500-2000
FC:1μg/Test
-
Fig1: Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).
Fig2: Sample: MCF-7 (human)cell Lysate at 40 ug
Primary: Anti- MARCH9at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38kD
Observed band size: 38 kD
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!