Anti-ST18 antibody
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概述
- 产品描述Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells.
- 产品名称Anti-ST18 antibody
- 分子量115 KDa
- 种属反应性Human,Mouse,Rat,Dog,Horse,Sheep
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human ST18/Znf387 451-550/1047
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Nucleus.
- 其它名称KIAA0535
St18
ST18_HUMAN
suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein)
Suppression of tumorigenicity 18 protein
Suppression of tumorigenicity protein 18
Zinc finger protein 387
Znf387.
more
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应用
WB:1:500-2000
FC:3ug/Test
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Fig1: Sample:
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- ST18at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kD
Fig2: Blank control: Mouse kidney.
Primary Antibody (green line): Rabbit Anti-ST18 antibody
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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