Anti-gamma tubulin antibody [10-A4]

Anti-gamma tubulin antibody [10-A4]

• 概述

• 产品描述Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.
• 产品名称Anti-gamma tubulin antibody [10-A4]
• 分子量51 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC
• 抗体类型小鼠单抗
• 免疫原Peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein G purified.
• 亚细胞定位Cytoplasm, cytoskeleton, centrosome.
• 其它名称
  • Gamma 1 tubulin antibody
  • Gamma 2 tubulin antibody
  • Gamma Tubulin 1 antibody
  more

• 应用

  WB: 1:1,000-1:5,000
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of gamma tubulin on different lysates using anti-gamma tubulin antibody at 1/2,000 dilution.
  Positive control:
  Lane 1: Jurkat
  Lane 2: Hela
  Lane 3: Human fetal brain tissue

   

  

  Fig2: ICC staining gamma tubulin (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining gamma tubulin (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining gamma tubulin (green) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-gamma tubulin antibody. Counter stained with hematoxylin.

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