Anti-StAR antibody

Anti-StAR antibody

• 概述

• 产品描述The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.
• 产品名称Anti-StAR antibody
• 分子量32 KDa
• 种属反应性Human,Mouse,Rat,Dog,Cow,Horse
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human StAR/StARD1 101-200/285
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Mitochondrion.
• 其它名称StARD1
  Cholesterol trafficker
  Luteinizing hormone induced protein
  Mitochondrial steroid acute regulatory protein
  StAR related lipid transfer (START) domain containing 1
  StARD1
  START domain containing protein 1
  Steroidogenic Acute Regulatory Protein
  Steroidogenic acute regulatory protein mitochondrial
  STAR_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:3μg /Test

•  

  

  Fig1: Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature).
  Primary Antibody (green line): Rabbit Anti-StAR antibody Dilution: 3μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.

   

  

  Fig2: Sample:
  Ovary (Mouse) Lysate at 30 ug
  Brain (Mouse) Lysate at 30 ug
  Primary: Anti-STAR/STARD1 at 1:200 dilution;
  Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
  Predicted band size : 32kD
  Observed band size : 32kD

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (StAR) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

   

  

  Fig4: Sample:
  Adrenal gland(mouse) Lysate at 40 ug
  Primary: Anti-StARat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 32kD
  Observed band size: 32 kD

   

  

  Fig5: Sample:
  K562(human) cell Lysate at 30 ug
  Primary: Anti-StAR  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 32kD
  Observed band size: 32 kD

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