Anti-CAMK4 antibody [B8-C5]

Anti-CAMK4 antibody [B8-C5]

• 概述

• 产品描述The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.
• 产品名称Anti-CAMK4 antibody [B8-C5]
• 分子量52 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus.
• 其它名称
  • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
  • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
  • Brain Ca++-calmodulin dependent protein kinase type IV antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CAMK4 on human CAMK4 recombinant protein using anti-CAMK4 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CAMK4 on HEK293 (1) and CAMK4-hIgGFc transfected HEK293 (2) cell lysate using anti-CAMK4 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CAMK4 on different cell lysate using anti-CAMK4 antibody at 1/1,000dilution.
  Positive control: Line1:Jurkat Line2:SK-N-SH Line3:Raji Line4:HeLa

   

  

  Fig4: ICC staining CAMK4 (green) and Actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-CAMK4 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CAMK4 antibody. Counter stained with hematoxylin.

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