Anti-Mesothelin antibody
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概述
- 产品描述This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. Mesothelin is over expressed in several human tumors, including mesothelioma, ovarian cancer, pancreatic adenocarcinoma and cholangiocarcinoma. The interaction between mesothelin and MUC16 (also known as CA125) may facilitate the implantation and peritoneal spread of tumors by cell adhesion. Dr. Mitchell Ho and colleagues at the National Cancer Institute identified the region (residues 296-359) consisting of 64 amino acids at the N-terminus of cell surface mesothelin as the functional binding domain for MUC16.
- 产品名称Anti-Mesothelin antibody
- 分子量45 kDa, cleavage form
- 种属反应性Human
- 验证应用WB,ICC,IHC-P
- 抗体类型兔多抗
- 免疫原Recombinant protein within Human Mesothelin aa 220-410.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/ml.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cell membrane, Golgi apparatus, Membrane, Secreted.
- 其它名称CAK 1 antibody
CAK1 antibody
CAK1 antigen antibody
cleaved form antibody
Megakaryocyte potentiating factor antibody
Mesothelin antibody
Mesothelin isoform 1 precursor antibody
MPF antibody
Msln antibody
MSLN_HUMAN antibody
Pre pro megakaryocyte potentiating factor antibody
Pre-pro-megakaryocyte-potentiating factor antibody
SMR antibody
SMRP antibody
Soluble MPF mesothelin related protein antibody
more
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应用
WB:1:500-1:2,000
ICC:1:50
IHC-P:1:50-1:200
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Fig1: Western blot analysis of Mesothelin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: Hela cell lysate
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Mesothelin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
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