Anti-Beta Actin antibody [A2-F6]

Anti-Beta Actin antibody [A2-F6]

• 概述

• 产品描述Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.
• 产品名称Anti-Beta Actin antibody [A2-F6]
• 分子量42 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, ICC, IHC-P, FC
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide (KLH-coupled) within human Beta-actin N terminal.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • A26C1A antibody
  • A26C1B antibody
  • ACTB antibody
  more

• 应用

  WB: 1:10,000
  ICC: 1:100-1:200
  IHC-P: 1:100-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution.
  Positive control:
  Lane 1: NIH/3T3 cell lysate
  Lane 2: PC12 cell lysate
  Lane 3: MCF-7 cell lysate
  Lane 4: HepG2 cell lysate
  Lane 5: Hela cell lysate
  Lane 6: Mouse lung tissue lysate

   

  

  Fig2: ICC staining of Beta-actin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining of Beta-actin in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining of Beta-actin in NIH-3T3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.

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