Anti-SIRP Alpha antibody

Anti-SIRP Alpha  antibody

• 概述

• 产品描述Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

• 产品名称Anti-SIRP Alpha antibody

• 分子量56 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CD172a 151-250/504

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Single-pass type I membraneprotein.

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• ​其它名称Signal regulatory protein alpha type 1
  BIT
  Brain Ig like molecule with tyrosine based activation motifs
  Brain immunoglobulin like molecule with tyrosine based activation motifs
  CD172 antigen like family member A
  CD172a
  CD172a antigen
  Inhibitory receptor SHPS1
  Macrophage fusion receptor
  MFR
  MYD 1
  Myd 1
  MYD1
  p84
  Protein tyrosine phosphatase non receptor type substrate 1
  PTPNS1
  SHP Substrate 1
  SHPS1
  Signal regulatory protein alpha 2
  Signal regulatory protein alpha 3
  Signal regulatory protein alpha
  Signal Regulatory Protein Alpha Type 1
  Signal regulatory protein alpha type 2
  SIRP
  SIRPA
  SIRPalpha
  SIRPalpha1
  SIRPalpha2
  SIRPalpha3
  Tyrosine phosphatase SHP substrate 1
  Tyrosine protein phosphatase non receptor type substrate 1
  Tyrosine protein phosphatase non receptor type substrate
  CD172a.

  more

• 应用

  WB:1:500-2000
  FC:1μg /test

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  Fig1: Sample:
  Spleen(Mouse) Lysate at 30 ug
  Bones (Mouse) Lysate at 30 ug
  Primary: Anti- SIRP Alpha  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 56 kD
  Observed band size: 53 kD

   

  

  Fig2: Blank control(blue): U937 (fixed with 2% paraformaldehyde (10 min)).
  Primary Antibody:Rabbit Anti- SIRP antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

   

  

  Fig3: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti-SIRP Alpha at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 56 kD
  Observed band size: 56 kD

   

  

  Fig4: Sample:
  Raw264.7(Mouse) Cell Lysate at 30 ug
  Primary: Anti-SIRP Alpha at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 56 kD
  Observed band size: 56 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！