Anti-FCGR1A/CD64 antibody
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概述
- 产品名称Anti-FCGR1A/CD64 antibody
- 分子量41 KDa
- 种属反应性Human,Mouse,Rat,Pig,Cow
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human CD64/IGFR1 101-200/374
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G.
- 其它名称CD 64
CD64 antigen
CD64A
Fc fragment of IgG high affinity Ia receptor
F Fc fragment of IgG, high affinity Ia, receptor (CD64)
Fc fragment of IgG, high affinity Ia, receptor for
Fc fragment of IgG, high affinity Ib, receptor (CD64)
Fc fragment of IgG, high affinity Ib, receptor for
Fc fragment of IgG, high affinity Ic, receptor (CD64)
Fc fragment of IgG, high affinity Ic, receptor for
Fc gamma receptor
Fc gamma receptor I A1
Fc gamma receptor I
Fc gamma receptor I B2
Fc gamma RI
Fc of IgG high affinity Ia receptor
Fc-gamma RIC
FCG 1
FCG1
FcgammaRI
FCGR 1
FCGR1
FCGR1A
FCGR1B
FCGR1C
FcRI
FCRIC
IGFR 1
IGFR1
IGFRB
IGFRC
IgG Fc receptor I
IgG Fc receptor IC
Immunoglobulin G Fc receptor I
Immunoglobulin G Fc receptor IB
Immunoglobulin G Fc receptor IC.
more
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应用
WB:1:500-2000
FC:1μg /test
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Fig1: Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-CD64 antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Fig2: Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti-FCGR1Aat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Fig3: Blank control: 293T Cells(blue).
Primary Antibody: Rabbit Anti-FCGR1A/CD64/AF647 Conjugated antibody ( Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Fig4: Blank control: Mouse spleen cells(blue).
Primary Antibody: Rabbit Anti- FCGR1A/CD64 antibody, Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody (were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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