Anti-CLGN antibody [G10-C8]

Anti-CLGN antibody [G10-C8]

• 概述

• 产品描述Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions.
• 产品名称Anti-CLGN antibody [G10-C8]
• 分子量70 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Endoplasmic reticulum membrane.
• 其它名称
  • 27 kDa interstitial collagenase antibody
  • CLG antibody
  • CLGN antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:100-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CLGN on human CLGN recombinant protein using anti-CLGN antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CLGN on HEK293 (1) and CLGN-hIgGFc transfected HEK293 (2) cell lysate using anti-CLGN antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CLGN on different cell lysate using anti-CLGN antibody at 1/1,000 dilution.
  Positive control: Line1:LNCaP Line2:HepG2 Line3:PC-3 Line4:Raji

   

  

  Fig4: ICC staining CLGN (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CLGN antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CLGN antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of HEK293 cells with CLGN antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！