Anti-ILDR1 antibody

Anti-ILDR1 antibody

• 概述

• 产品描述Putative membrane receptor.
• 产品名称Anti-ILDR1 antibody
• 分子量60 KDa
• 种属反应性Human,Mouse,Rat,Pig,Cow,Horse,Rabbit,Sheep
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human ILDR1 101-200/546
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane; Single pass type I membrane protein. Isoform 5: Cytoplasm (cytosol).
• 其它名称ILDR1alpha
  ILDR1beta
  Immunoglobulin like domain containing receptor 1 alpha
  Immunoglobulin like domain containing receptor 1 alpha
  Immunoglobulin like domain containing receptor 1
  Immunoglobulin like domain containing receptor 1 beta
  MGC50831
  ILDR1_HUMAN.
  more

• 应用

  WB:1:500-2000
  FC:3ug/Test

•  

  

  Fig1: Blank control: A431.
  Primary Antibody (green line): Rabbit Anti-ILDR1 antibody
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody: Goat anti-rabbit IgG-AF647
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

   

  

  Fig2: Sample:
  DU145(Human) Cell Lysate at 30 ug
  Primary: Anti-ILDR1  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 60 kD
  Observed band size: 56 kD

   

  

  Fig3: Sample:
  Prostate(Rat) Cell Lysate at 40 ug
  Primary: Anti-ILDR1 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 60 kD
  Observed band size: 56 kD

   

  

  Fig4: Sample:
  Pancreas(Mouse) Cell Lysate at 40 ug
  Primary: Anti-ILDR1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 60 kD
  Observed band size: 56kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！