Anti-NPY2R / Y2 receptor antibody
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概述
- 产品描述Neuropeptide Y (NPY) and its family of five receptors are known to be associated with hypothalamic regulation of feeding behavior, metabolism, and energy homeostasis in both rodents and humans. In particular, knock-out mice for the gene neuropeptide Y 2 receptor, NPY2R, exhibit hyperphagia and excessive weight gain. As a result, research on the gene has focused on genotyping analysis for the identification of single nucleotide polymorphisms (SNPs) in obese human subjects and drug development for manipulation of its expression as a novel therapeutic treatment for obesity. Although primarily expressed in the arcuate nucleus of the hypothalamus, NPY2R may also be found in other tissues including trabecular bone, vascular tissue, and colonic mucosa.
- 产品名称Anti-NPY2R / Y2 receptor antibody
- 分子量43 kDa (Predicted band size)
- 种属反应性Human
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within Mouse NPY2R / Y2 receptor aa 341-369.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Membrane.
- 其它名称Neuropeptide Y receptor type 2 antibody
Neuropeptide Y receptor Y2 antibody
NPY-Y2 receptor antibody
NPY2-R antibody
NPY2R antibody
NPY2R_HUMAN antibody
Y2 receptor antibody
more
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应用
WB:1:500-1:1000
FC:1:50-1:100
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Fig1: Western blot analysis of NPY2R / Y2 receptor on MCF-7 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Flow cytometric analysis of NPY2R / Y2 receptor was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody(red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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