Anti-Bcl-2 antibody
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概述
- 产品描述Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments. Antibodies to Bcl-2 can be used with immunohistochemistry to identify cells containing the antigen. In healthy tissue, these antibodies will react with B-cells in the mantle zone, as well as some T-cells. However, there is a considerable increase in positive cells in follicular lymphoma, as well as many other forms of cancer. In some cases, the presence or absence of Bcl-2 staining in biopsies may be significant for the patient's prognosis or likelihood of relapse.
- 产品名称Anti-Bcl-2 antibody
- 分子量26kDa
- 种属反应性Human
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide (KLH-coupled) within human Bcl-2 aa 20-80.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Mitochondrion outer membrane, nucleus membrane
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应用
WB: 1:500
ICC: 1:200
IHC-P: 1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of Bcl-2 on different cell lysates using anti-Bcl-2 antibody at 1/500 dilution.
Fig2: ICC staining Bcl-2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Bcl-2 rabbit polyclonal antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 rabbit polyclonal antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of Hela cells with Bcl-2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
