Anti-PRDM1 antibody [B6-F1]

Anti-PRDM1 antibody [B6-F1]

• 概述

• 产品描述The development and differentiation of plasma cells, which are terminally differentiated B-cells, are induced by Blimp-1 (B lymphocyte-induced maturation protein, also designated PRDI-BF1). Blimp-1 is a transcriptional repressor that localizes to the nucleus and is considered a master regulator of terminal B-cell development. Alone, Blimp-1 is sufficient to trigger terminal B-cell differentiation. Blimp-1 upregulates the expression of syndecan-1 and J chain, represses IFN-b gene transcription and associates with HDAC to recruit it to DNA, thereby repressing c-myc. Blimp-1 is expressed during the late stages of B-cell differentiation in immunoglobulin-secreting plasma cells, as well as in long-lived, bone marrow plasma cells. The expression of Blimp-1 defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated.
• 产品名称Anti-PRDM1 antibody [B6-F1]
• 分子量92 kDa
• 种属反应性Human
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Cytoplasm.
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• 其它名称
  • B Lymphocyte Induced Maturation Protein 1 antibody
  • Beta interferon gene positive regulatory domain I binding factor antibody
  • Beta-interferon gene positive regulatory domain I-binding factor antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:200-1:1,000
  FC: 1:100-1:200

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  Fig1: Western blot analysis of PRDM1 on human PRDM1 recombinant protein using anti-PRDM1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PRDM1 on HEK293 (1) and PRDM1-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM1 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining PRDM1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Flow cytometric analysis of Raji cells with PRDM1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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